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KMID : 0900919980220010095
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Korean journal of Animal Reproduction
1998 Volume.22 No. 1 p.95 ~ p.99
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A Study on Culture Environments of In Vitro Matured/In Vitro Fertilized Bovine Embryos I. Influence of Somatic Cells, Growth Factors or Culture Media on In Vitro Maturation of Bovine Oocytes
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Lee Myung -Shik
Park Jin-Ki
Min Kwan-Sik
Baek Kwang-Soo
Seong Hwan-Hoo
Park Y.-Y.
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Abstract
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Three experiments were conducted with follicular oocytes, to compare some somatic cells, growth factors and media for in vitro maturation of bovine oocytes. In the first experiment, the type of somatic cells had no effects on in vitro maturation of bovine follicular ooctyes. In the second experiment, oocytes were matured in TCM199 su, pp.emented with growth factors on IVM of bovine follicular oocytes, then all were co-cultured with cumulus cells. The proportion of used oocytes that developed to expanding blastocysts was 22.2%, 20.2%, 17.7%, 22.2%, 24.4% and 20.2% after maturation in TCM199 su, pp.emented with control, insulin, IGF-I, IGF-Ⅱ, FGF and EGF, respectively. In the third experiment, oocytes were matured in BO, Ham’s F10 and TCM199, then all were fertilized in BO, and embryos cultured in BO, Ham’s F10 and TCM199, respectively. Cleavage rates in BO were 90%, had higher than in Ham’s F10(80%) or in TCM199(64%). But production of expanding blastocysts in TCM199(21%) or Ham’s F10(20.6%), had higher than in BO(4.6%).
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KEYWORD
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Oocyte, Maturation : Somatic cell, Growth factor, Media
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